The antiinflammatory effects of AF-1 on bleomycin-induced lung injury. (A). Experimental design 2: AF-1 (2 mg · kg-1 · day-1) was injected intraperitoneally from D0 to D6. On D7, mice were killed and lung samples were collected for further analysis. The degree of acute lung injury was assessed by H&E staining (×200, Scale bar = 100 μm) (B) and W/D ratio (C). The levels of TNF-α (D) and IL-1β (E) in lung homogenate were quantified by ELISA. n = 5–11. Bars: median values (TNF-α and IL-1β) or mean ± SD (W/D). *P ≤ 0.05 vs BLM group.