Figure 5
Expression pattern of hypoxia-induced mitogenic factor (HIMF) following chronic hypoxia or Ova challenge. (A) Bronchioalveolar lavage fluid (BALF) and (B) whole-lung homogenates from normoxic (4 days, 20.8% O2), hypoxic (4 days, 10.0% O2), saline-challenged, and Ova-challenged mice were resolved by 4–20% SDS-PAGE and transferred to nitrocellulose. The blots were probed with rabbit anti-HIMF antibodies and developed using enhanced chemiluminescence. To confirm equal loading and transfer, the blots were stripped and reprobed with β-actin monoclonal antibodies. IB, immunoblot; IB*, immunoblot after stripping. (C) Paraffin-embedded lung sections from normoxic, hypoxic, saline-challenged, and Ova-challenged mice were rehydrated and stained with antibodies raised against HIMF (brown). Sections were counterstained with hematoxylin (blue). Aw: airway. Arrows: small pulmonary vessels. Scale bar: 50 μm.