Figure 6

Effect of guaifenesin (GGE), N -acetylcysteine (NAC) or ambroxol (Amb) on mucociliary transport rates. Individual particle rates for endogenous particles on the surface of human primary lung epithelial cell cultures are indicated by symbols. Lighter coloured symbols with black borders indicate the 30 μM concentration of the drugs, while darker solid symbols indicate the 100 μM concentration of the drugs. Target data collection was for at least 5 moving elements from each of three fields on each of 4 cultures. In some cases, insufficient numbers of particles or fields with particles were found. Panels on the left side indicate the combined data from elements subjectively designated as individual particles or those associated with mucus sheets. The middle panels show only those designated as individual particles and the panels on the right show only those associated with mucus sheets. *, ** and *** indicate p < 0.05, 0.01 and 0.005, respectively, vs. the IL-13-only control (ANOVA with Dunnett’s post-test). Horizontal lines above the groups indicate trend tests with p values < 0.05 (all include the IL-13-alone, zero drug, condition as indicated by the short line over this bar at the same height).