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Figure 5 | Respiratory Research

Figure 5

From: Effects of guaifenesin, N-acetylcysteine, and ambroxol on MUC5AC and mucociliary transport in primary differentiated human tracheal-bronchial cells

Figure 5

Effect of guaifenesin (GGE), N -acetylcysteine (NAC) or ambroxol (Amb) on cellular MUC5AC content. The MUC5AC content in lysates of quadruplicate cultures for each condition were analysed by ELISA. No dithiothreitol was used in this ELISA because the lysis buffer did not contain this reducing agent. The results are reported as concentration relative to the standard curve, generated from dilutions of a pooled sample of washes collected from other samples. Separate cultures were analysed at the three time points. *, ** and *** indicate p < 0.05, 0.01 and 0.005, respectively, vs. the IL-13-only group (ANOVA with Dunnett’s post-test). Horizontal lines above the groups indicate significant trend tests (all include the IL-13-alone, zero drug, condition as indicated by the short line over this bar at the same height).

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