Figure 5

The effects of ciglitazone (CTZ) and rosiglitazone (RTZ) on cytokine-induced CXCL10 release by asthmatic (A) and non-asthmatic (NA) ASMC. Confluent serum-deprived ASMC were treated with vehicle or A) ciglitazone for 0.5 h and then stimulated with the cytokines (10 ng/ml each) IL-1β, TNF-α or IFNγ, or B) and C) the PPARγ antagonist GW9662 for 0.5 h prior to and during treatment with B) ciglitazone or C) rosiglitazone for 0.5 h followed by stimulation with cytomix (IL-1β, TNF-α and IFNγ combined, each at 10 ng/ml). CXCL10 release after 24 h cytokine stimulation was quantified.