Leaky lysosomes in lung transplant macrophages: azithromycin prevents oxidative damage

Respiratory Research

Table 3 The influence of different 4-hr treatments (for details see Methods section) on cell features that are decisive for lysosomal Fe ²⁺ -reactivity in murine 774 macrophages

*ASSAYS*	Cont.	AZM (μM)		H-DFO (mM)	NH₄Cl (mM)	FeCl₃ (μM)
		33	660	2	10	100
Golde Index (n = 3)	0.7 ± 0.8	0.6 ± 0.7	0.5 ± 0.5	0.2 ± 0.4	0.4 ± 0.6	194.3 ± 6.1***
Cellular iron (ng/mg protein) (n = 4)	0.2 ± 0.1	0.2 ± 0.0	0.2 ± 0.0	0.2 ± 0.0	0.2 ± 0.0	16.2 ± 2.3***
H-ferritin (ng/mg protein) (n = 3)	6.9 ± 1.4	8.2 ± 2.4	8.6 ± 1.7	7.6 ± 1.9	6.0 ± 1.8	109.8 ± 9.7**
GSH (ng/mg protein) (n = 4)	21.3 ± 2.0	20.7 ± 1.6	23.2 ± 3.3	20.2 ± 2.0	26.3 ± 3.9	26.3 ± 3.8*

To evaluate the different assays, the analysis was also performed on J774 macrophages that were exposed to 100 μM FeCl₃ for 16 h. Data are presented as the means ± 1 SD. *, ** and *** indicate a significant difference from untreated control cells of p < 0.05, p < 0.01 and p < 0.001, respectively.

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