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Table 3 The influence of different 4-hr treatments (for details see Methods section) on cell features that are decisive for lysosomal Fe 2+ -reactivity in murine 774 macrophages

From: Leaky lysosomes in lung transplant macrophages: azithromycin prevents oxidative damage

ASSAYS Cont. AZM (μM) H-DFO (mM) NH4Cl (mM) FeCl3 (μM)
   33 660 2 10 100
Golde Index (n = 3) 0.7 ± 0.8 0.6 ± 0.7 0.5 ± 0.5 0.2 ± 0.4 0.4 ± 0.6 194.3 ± 6.1***
Cellular iron (ng/mg protein) (n = 4) 0.2 ± 0.1 0.2 ± 0.0 0.2 ± 0.0 0.2 ± 0.0 0.2 ± 0.0 16.2 ± 2.3***
H-ferritin (ng/mg protein) (n = 3) 6.9 ± 1.4 8.2 ± 2.4 8.6 ± 1.7 7.6 ± 1.9 6.0 ± 1.8 109.8 ± 9.7**
GSH (ng/mg protein) (n = 4) 21.3 ± 2.0 20.7 ± 1.6 23.2 ± 3.3 20.2 ± 2.0 26.3 ± 3.9 26.3 ± 3.8*
  1. To evaluate the different assays, the analysis was also performed on J774 macrophages that were exposed to 100 μM FeCl3 for 16 h. Data are presented as the means ± 1 SD. *, ** and *** indicate a significant difference from untreated control cells of p < 0.05, p < 0.01 and p < 0.001, respectively.