Surfactant Protein D modulates allergen particle uptake and inflammatory response in a human epithelial airway model

Table 2 Secretion of cytokines and chemokines after 8 hours incubation with subpollen particles (SPP) plus surfactant protein d (SP-D) and further 72 h incubation with fresh medium in the presence of T-cells

	Cells	10 × 10⁶ SPP	10 × 10⁶ SPP + 1 μg/ml SP-D	10 × 10⁶ SPP +10 μg/ml SP-D	10 μg/ml SP-D
IL-1 alpha	108.2 ± 22,0	170.6 ± 40.6*	149.6 ± 33.7	192.7 ± 57.4	102.0 ± 25.6
G-CSF	1853 ± 434.3	2823 ± 623.2	2665 ± 566.7	2534 ± 528.2	2596 ± 560.8
TNF-alpha	150.9 ± 43.2	339.4 ± 118.5	250.2 ± 102.4	268.8 ± 103.3	235.3 ± 128.6
MIP-1beta	1211 ± 105.6	1408 ± 143.6*	1297 ± 233,5	1448 ± 183.4	1224 ± 190.0
IL-6	1119 ± 295.9	2327 ± 577.3	1717 ± 284.8	1812 ± 1388.5	1051 ± 151.7

Values are shown as means of at least 8 experiments ± SEM and are given in pg/ml. Detection limit was 3.2 pg/ml. Limit of quantification was: IL-1 alpha 80 pg/ml; G-CSF 80 pg/ml; TNF alpha 80 pg/ml; MIP-1 beta 80 pg/ml; IL-6 16 pg/ml; * p < 0.05 vs Cells

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