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Figure 1 | Respiratory Research

Figure 1

From: Functional characterization of the matrix metalloproteinase-1 cigarette smoke-responsive region and association with the lung health study

Figure 1

Sp1 action on the MMP-1 promoter is shown by the fold change in specific DNA sequences detected by qPCR. MMP-1 ChIP assays were done in cell cultures from human small airway epithelial cells with and without cigarette smoke extract (CSE) treatment using an antibody to Sp1 or nonimmune serum. We amplified DNA using primers spanning two Sp1 binding sites within the cigarette smoke-responsive element (a,b) and one sequence outside of this element (c). Samples from each experiment were run in triplicate. Data are mean ± SEM. *P < 0.05.

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Respiratory Research

ISSN: 1465-993X

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