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Figure 4 | Respiratory Research

Figure 4

From: Hypoxia-induced DNA hypermethylation in human pulmonary fibroblasts is associated with Thy-1 promoter methylation and the development of a pro-fibrotic phenotype

Figure 4

The Thy-1 promoter becomes hemimethylated in hypoxic pulmonary fibroblasts. Human pulmonary fibroblasts were grown in hypoxia for 4 and 8 days. Genomic DNA was bisulfite treated and methylation specific PCR (MSPCR) using unmethylated (U) and methylated (M) specific primers were used to investigate the methylation status of the Thy-1 promoter. MSPCR revealed Thy-1 promoter hemimethylation in hypoxic cells (n = 3) that was not present in normoxic controls (n = 3).

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Respiratory Research

ISSN: 1465-993X

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