Figure 3From: Hypoxia-induced DNA hypermethylation in human pulmonary fibroblasts is associated with Thy-1 promoter methylation and the development of a pro-fibrotic phenotypeHypoxic human pulmonary fibroblasts express decreased levels of Thy-1 mRNA. CCD19Lu were grown for 4 and 8 days in 1% oxygen and expression of Thy-1 was quantified using quantitative real time PCR. There was a significant reduction in Thy-1 expression in hypoxic cells after 4 and 8 days, p < 0.05 (*) and p < 0.01 (**), respectively, compared to normoxic control expression. Results are mean +/- SD for n = 4 experiments and students t test was used for statistical analysis.Back to article page