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Figure 1 | Respiratory Research

Figure 1

From: Hypoxia-induced DNA hypermethylation in human pulmonary fibroblasts is associated with Thy-1 promoter methylation and the development of a pro-fibrotic phenotype

Figure 1

Hypoxia causes global hypermethylation in human pulmonary fibroblasts. CCD19Lu were grown for 4 and 8 days in 1% O2 and levels of DNA methylation were measured using an antibody directed towards 5 methylcytosine (5MeC) and quantitative flow cytometry. Hypoxic cells had significantly increased levels of 5MeC, measured as mean channel fluorescence (MCF), compared to levels in normoxic cells. Results are mean +/- SD for n = 3 experiments and statistical analysis was performed using one way anova where p < 0.05 (*) and p < 0.01 (**).

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Respiratory Research

ISSN: 1465-993X

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