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Figure 3 | Respiratory Research

Figure 3

From: Nitric oxide-induced eosinophil apoptosis is dependent on mitochondrial permeability transition (mPT), JNK and oxidative stress: apoptosis is preceded but not mediated by early mPT-dependent JNK activation

Figure 3

SNAP induces late loss of ΔΨ m . Eosinophils were incubated with solvent, SNAP (1 mM), penicillamine (1 mM) and/or GM-CSF (10 pM) for indicated times (A) or for 40 hours (B-C) after which JC-1-staining and analysis of mitochondrial membrane potential by flow cytometer was conducted as described in materials and methods. In A shown are mean ± SEM from five independent experiments. * indicates p < 0.05 and ** p < 0.01 when compared to cells treated with GM-CSF or GM-CSF and penicillamine, respectively. In B-C shown are representative graphs from analysis of ΔΨm with percentages of cells with lost ΔΨm.

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