Skip to main content
Figure 2 | Respiratory Research

Figure 2

From: Nitric oxide-induced eosinophil apoptosis is dependent on mitochondrial permeability transition (mPT), JNK and oxidative stress: apoptosis is preceded but not mediated by early mPT-dependent JNK activation

Figure 2

SNAP-induced apoptosis is dependent on late mPT but preceded by early partial mPT. Bongkrekic acid (62 μM) or solvent was added either 2 h before (A-H) or 16 h (H) after 10 pM GM-CSF, 1 mM SNAP and/or 1 mM penicillamine. In A-D Eosinophils were incubated for 1 h after which mPT was determined by calcein AM/CoCl2-method as described in materials and methods. In A-C shown are representative graphs from analysis of mPT and in the top right corner percentage of calcein fluorescence of its initial value describing the extent of mPT. In E-H total incubation time of eosinophils was 40 h after which apoptosis was determined by DNA fragmentation assay (E, H), morphological analysis (F) or Annexin-V FITC staining (G). Values are mean ± SEM from five to six independent experiments, * indicates p < 0.05 and ** p < 0.01 as compared with the respective control.

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us