Figure 7

ERK and Akt activation was inhibited by HSULF-1 but restored by heparin in H292 cells. Cells were infected with lacZ or HSULF-1 adenovirus at 10 MOI, cultured for 48 hours, and exposed to 25 μg/ml heparin for 5 minutes. Top, Western blot analysis (A). Bottom, Densitometric analysis of p-ERK (B) and p-AKT (C). Integrated optical density values were analyzed using Image J, normalized to total ERK and Akt, and expressed as percentage of Control with means ± SD from 3 experiments. * Significant differences at p<0.05 compared to control cells, as determined by t-test.