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Figure 5 | Respiratory Research

Figure 5

From: HSULF-1 inhibits ERK and AKT signaling and decreases cell viability in vitro in human lung epithelial cells

Figure 5

TUNEL assay of H292 cells over-expressing HSULF-1. Cells were infected with lacZ adenovirus alone (control, A) or HSULF-1 adenovirus at 2, 5, 10, 20, 50, and 100 MOIs (sequentially increasing, B – G). Seventy two hours post-infection, TUNEL assay was performed on harvested cells. Photomicrographs were taken of random fields and a minimum of 3 fields from photomicrographs were selected and total cells were counted in each field to achieve a minimum number of 150 total cells. (H) Apoptotic ratios as apoptotic cells/total cells are expressed as Mean ± SD from different fields. Significant differences between groups at p<0.05 as determined by Student’s t-test are indicated by the letters above each bar, with different letters indicating significant differences between groups.

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