Figure 2

Demonstration of HSULF-1 over-expression in hAT2 and H292 cells by quantitative real time PCR (qRT-PCR) and Western blot. (A) Cells were infected with HSULF-1 adenovirus at MOIs from 5 to 100, with lacZ adenovirus at 100 MOI serving as a control. 48 hours post-infection, qRT-PCR was performed to assess the expression level of HSULF-1 mRNA. Values are shown as mean ± SD from 2 experiments. * Significant differences at p<0.05, compared with untreated and lacZ controls, are determined by Student’s t-test. Western blot was utilized to assess the expression level of HSULF-1 protein 72 hours post-infection in (B) hAT2 cells and (C) H292 cells.