Figure 1

Relative levels of baseline HSULF-1 expression. Five normal cells (HFL-1, 16Lu, hAT2, HBE, and HLF) and five cancer cell lines (A549, H292, H1975, H1703, and H661) were cultured for 48 hours and the levels of HSULF-1 mRNA were analyzed by RT-PCR. Values were normalized to HSULF-1 expression in A549 cells (lowest expression), log transformed to obtain normal distribution, and expressed as means ± SD from three separate analyses. All the cancer cells express HSulf-1 in a significantly lower level (*) than all normal cells at a p-value of 0.0004 as determined by one tailed Student’s t-test.