Figure 5

CpG ODN treatment decreases IL-17A and TGF-β1 production. Mice were treated as described in Figure 2. A) Cells were isolated from the BAL and draining thoracic lymph nodes 3 days after BLM instillation. These cells were stimulated in vitro with 50 ng/ml PMA plus 1 μg/ml ionomycin for 4 hours. The concentration of IL-17A in culture supernatants was determined by ELISA. B) The concentration of TGF-β1 in BAL fluid collected 3 days after BLM instillation was determined by ELISA. Data show the change in concentration of IL-17A and TGF-β1 (avg + SE) as a percentage of the BLM treated group alone from 2-3 independent experiments involving 9-14 mice/group. **; p < 0.005.