Effect of CpG ODN on BLM-induced leukocyte accumulation in the lungs. Mice were injected i.p. with 200 μg of control or CpG ODN 5 days before 0.05 U of BLM was delivery intra-tracheally. A) The number of leukocytes present in BAL collected 1 - 7 days after BLM instillation was determined. Statistic significance was analyzed by longitudinal regression analysis. B) Cell differentials (based on 300 leukocytes/sample) in BAL collected 5 days after BLM instillation were performed on cytocentrifuge preparations after Diff-Quick staining. Data show the mean ± SE of the combined results from 2 independent experiments involving 10 mice/group/time point. **; p < 0.005.