Figure 3
From: Rituximab therapy in pulmonary alveolar proteinosis improves alveolar macrophage lipid homeostasis
Intracellular lipid staining is decreased post rituximab therapy in PAP alveolar macrophages. Cytospin preparations of alveolar macrophages were stained with Oil Red O and intensity was quantified using a modified scoring previously described [21, 23]; (+++ = strongly positive, ++ = positive; + = weakly positive. A minimum of 100 cells on cytospins from each of 5 paired pre and post therapy samples were scored.