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Figure 7 | Respiratory Research

Figure 7

From: Nrf2 protects human alveolar epithelial cells against injury induced by influenza A virus

Figure 7

AdNrf2 decreases IAV infection in ATI-like cells. Cells were infected at a MOI of 200 pfu/cell AdNrf2 followed by infection at a MOI of 1 pfu/cell PR8 virus for 24 h. Panel I – Oxidative stress was measured by immunocytofluorescence with an antibody to 4-HNE as described in the Methods section. a - control; b – AdGFP, c - AdNrf2; d – PR8 virus; e - AdNrf2 and PR8 virus. Panel II – Infection with PR8 virus significantly increased influenza A nucleoprotein and HO-1 expression. Infection with AdNrf2 followed by PR8 virus decreased their expression (immunoblotting). Lane 1- control; lane 2 – AdGFP; lane 3 - AdNrf2; lane 4 – PR8 virus; and lane 5 - AdNrf2 and PR8 virus. Relative expression of these proteins is also shown. Panel III – AdNrf2 decreased replication of PR8 virus in comparison with PR8 alone. Cells were infected with IAV or AdNrf2 followed by infection with PR8 virus. Media were harvested 24 h after inoculation and titrated by plaque assay in MDCK cells as described in the Methods section. Panel IV – IAV increases antiviral gene Mx1 and OAS1 mRNA levels as measured by RT-PCR. Infection with AdNrf2 followed by PR8 virus significantly decreased their expression. * Statistically significant increase in the gene expression in comparison with control. # Statistically significant decrease in comparison with PR8 virus alone (Mean ± SEM, N = 3, p < 0.05).

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