Figure 6

AdNrf2 protects ATI-like cells against injury by PR8 virus. ATI-like cells were infected for 24 h at a MOI of 200 pfu/cell AdNrf2 or AdGFP as described in the Methods section. Panel I – Nrf2 was overexpressed by AdNrf2 and cells were harvested at 24 hpi: Lane 1 - control; lane 2 - AdNrf2, lane 3 - AdGFP (immunoblotting). Relative expression of this protein is also shown. Panel II – Cells were infected with AdNrf2 followed by infection at a MOI of 0.05, 0.5 and 1 pfu/cell IAV for 24 h. A higher percentage of necrotic cells was observed in cells infected with PR8 virus in comparison with cells infected with AdNrf2 and followed by infection with PR8 as detected by Hoechst 33342 and propidium iodide double staining. * Statistically significant increase in the percentage of necrotic cells by PR8 infection in comparison with control. # Statistically significant decrease of ATI-like necrotic cells after infection with AdNrf2 and PR8 in comparison to PR8 virus alone (Mean ± SEM, N = 3, p < 0.05). Data represent results from three independent experiments.