Figure 3

AM ingest PR8 virus-induced apoptotic ATII cells. ATII cells were infected with IAV and floating apoptotic cells were collected after 48 h. Floating cells (red) were separately fluorescently stained and added to labeled AM (green) as described in the Methods section. a – AM alone; b – apoptotic floating ATII cells induced by a MOI of 1 pfu/cell PR8 virus after 48 h; c – ingestion of PR8 virus-induced apoptotic ATII cells by AM; d – percent of efferocytosis of viable ATII cells and PR8 virus-induced apoptotic ATII cells by AM. Data represent results from three independent experiments (Mean ± SEM, * p < 0.05).