Figure 10
From: Nrf2 protects human alveolar epithelial cells against injury induced by influenza A virus
AdNrf2 protects cells and Nrf2 siRNA sensitizes cells to injury induced by PR8 virus. ATI-like cells (a) and ATII cells (b) were grown as described in the Methods section and infected with PR8 virus at a MOI of 0.5 pfu/cell for 24 h. Panel I, cell infection with AdNrf2 followed by infection with PR8 virus decreased ROS generation in comparison with PR8 alone as measured by Amplex Red kit. Cell transfection with Nrf2 siRNA followed by infection with PR8 virus increased ROS production in comparison with PR8 alone. * Statistically significant difference in comparison with control. # Statistically significant decrease in comparison with PR8 virus alone. & - Statistically significant increase in comparison with PR8 virus alone. Data represent results from three independent experiments. Panel II, AdNrf2 increased GSH level in comparison with control. Cell transfection with Nrf2 siRNA followed by infection with PR8 virus decreased GSH level in comparison with PR8 virus alone. * Statistically significant difference in comparison with control. # Statistically significant increase in comparison with PR8 virus alone. & - Statistically significant decrease in comparison with PR8 virus alone. Data represent results from three independent experiments (Mean ± SEM, * p < 0.05).