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Table 1 Cytokine concentration (pg/ml) in cell culture supernatant following in vitro K. pneumoniae infection of Alveolar Macrophages

From: Inhaled fluticasone propionate impairs pulmonary clearance of Klebsiella Pneumoniae in mice

Vehicle-Exposed AM IL-6 IP-10 KC LIX MCP-1 MIP-1a MIP-1b MIP-2 TNFa
PBS 5.54 (4.54) 43.96 (2.44) 166.47 (11.12) 79.93 (15.99) 61.07 (5.78) 68.53 (4.30) 26.05 (8.24) 1,225.18 (36.94) 52.07 (0.93)
Klebseilla 2,506.37 (167.12) 122.53 (5.10) 13,917.04 (198.04) 1,518.32 (57.78) 68.74 (2.19) 10,038.19 (180.37) 9,318.00 (1,764.90) 41,802.27 (6,019.32) 2,012.61 (724.25)
Klebs + 10 nm FP 661.80* (36.50) 125.74 (3.51) 8,254.84* (268.01) 931.97* (21.59) 47.11* (1.71) 7,731.08* (161.64) 3,795.81* (200.74) 11,881.55* (622.15) 1,141.86 (100.01)
Klebs + 100 nm FP 472.29*# (23.18) 116.16 (6.96) 7,912.98* (271.24) 818.89* (22.82) 35.31*# (1.13) 6,674.62*# (252.86) 3,252.32*# (82.53) 11,441.20 (736.01) 1,101.10 (62.84)
FP-Exposed AM          
PBS 1.00 (0) 8.99 (1.23) 84.31 (1.72) 2.59 (1.59) 43.29 (5.49) 34.76 (2.98) 3.54 (2.54) 520.03 (26.90) 19.73 (0.85)
Klebseilla 828.87 (85.73) 122.38 (10.04) 9,891.14 (525.77) 1,056.19 (55.29) 50.38 (2.22) 9,111.51 (146.05) 5,640.23 (776.27) 14,296.83 (700.94) 1,096.82 (115.99)
Klebs + 10 nm FP 354.74* (23.31) 105.06 (5.47) 7,472.76* (459.38) 746.89* (45.01) 33.22* (1.35) 6,636.20* (378.28) 3,670.30* (228.33) 10,762.57* (557.97) 1,066.93 (71.74)
Klebs + 100 nm FP 327.28* (27.06) 107.73 (5.09) 6,492.42 (130.07) 701.09* (44.26) 25.64*# (0.90) 5,610.73*# (188.29) 3,511.53* (121.10) 8,612.62*# (355.80) 1,029.21 (122.45)
  1. Cytokine expression by vehicle and FP-treated murine AM 4 h after in vitro stimulation with K. pneumoniae. The top four rows show mean and (SEM) cytokine responses by AM lavaged from vehicle treated mice, and bottom four rows are for AM from FP-treated mice. Some groups were also pre-treated in vitro for 2 h with FP (10 or 100 nM) before infection. Results suggest a dose-dependent effect of in vitro FP exposure on AM recovered from either naïve or FP nebulized mice. n = 6 per group. * p < 0.05 vs. Klebsiella exposure group; # p < 0.05 vs. Klebsiella + 10 nM FP group.