Inhibition of tyrosine phosphorylation of Pyk2 by TAT-Pyk2-CT in lung tissue. Mice were treated intraperitoneally with 10 mg/kg TAT-Pyk2-CT or TAT-GFP 1 h prior to LPS challenge. Mice were sacrificed 18 h after LPS challenge and the lungs were removed. Lung lysates were analyzed for phosphorylated Pyk2 at Y402 using Western blot analysis. Upper panel is the representative immunoblot from three experiments. The lower panel is the relative optical density of phosphorylated Pyk2 normalized against corresponding total Pyk2 from three blots. #P < 0.05 vs. saline control; *P < 0.05 vs. LPS alone group.