Figure 1

ADRB2 3΄UTR poly-C repeat region. (A) Structural overview of the ADRB2 gene, detailing the common amino acid changing SNPs, and flanking variants (↓). Annotated are the ADRB2 upstream peptide (BUP), ATG start codon, coding region (shaded), 3′ untranslated region (3'UTR), and polyA addition signal. (B) Expanded view of the ADRB2 3′UTR immediately downstream of the stop codon. SNPs located at the end of the coding sequence, as well as those located within the poly-C repeat region, are shown (↓). 3′UTR SNPs identified in this study are underlined. The C nucleotide that is mismatched in the sequencing primer to a T nucleotide (see Methods) is highlighted in larger bold font (position +1269). Below the sequence, arrows indicate the position of the primers described in the text (see Methods). Sizes of the PCR products, including sequencing tags, are shown at the right-hand side. (C) An expanded view showing detail of the variable nature of the ADRB2 3'UTR. The poly-C repeat region (rs45580732) is boxed with internal SNPs highlighted by shaded nucleotide bases. The location of the mismatch primer is indicated by the dotted lines expanding out the arrow in Figure 1b. ★The C > G SNP at position +1268 was previously described by Hawkins et al. (2006) [16]. ► indicates novel variants identified in this study. SNP rs IDs enclosed in dashed boxes below the table are database variants that were not seen among the chromosomes sequenced in this study.