Figure 8

Cellular composition and in vitro proliferative capacities of PAEC and PASMC from (sub)segmental pulmonary arteries of CTEPH patients. Serial contiguous sections of (sub)segmental pulmonary arteries from CTEPH patients were stained with antibodies raised against α-SMA (A) or CD31 (B). Nuclei were counterstained using DAPI (blue). Magnification: main image, 40 × (bar, 50 μm); insert, 20 × (bar, 100 μm). Subconfluent cells were starved for 24 h in 0.2% FBS medium. Mitogenic activity was measured in the presence of 0.2% (control) or 10% FBS (C) or 5% FBS (D) as described above. (D) *p = 0.03.