Figure 3

T cell alloreactivity in response to cDC or pDC stimulation. Lung transplantation was performed as described in Methods and transplanted lungs harvested at 7 days post transplant. A-D) Mediastinal lymph node CD3+ T cells from lung transplant recipient (C57BL/6) mice were cultured alone (3 × 10⁵) or in the presence of Wt donor (BALB/c)-derived cDCs or pDCs (3 × 10⁴). Cells were incubated for 72 h at 37°C. T-cell proliferation was measured by 3H thymidine incorporation. A) Spontaneous T cell proliferation of Wt, DTR, PDCA1 and DTR/pDCA1 transplant groups. B) cDC and pDC were co-cultured with Wt T cells isolated from lung transplant recipients that received dendritic cell sufficient lung allografts. C) cDC and pDC co-cultured with recipient-derived T cells isolated from cDC, pDC or cDC/pDC depleted transplant groups. D) pDC and cDC co-cultured with recipient-derived T cells isolated from cDC and pDC depleted transplant groups. Responses of recipient cells were compared using one-way ANOVA analysis. ** p < 0.01, # p = 0.01, ## p = 0.02 * p < 0.05 Panels E-H represent H&E stained lung sections from allografts harvested at day 7. Table 2) Histologic rejection scores for transplant groups. Data represent the mean +/- SD of "A" scores for four to six mice in each group harvested at 7 days. *p < 0.05 compared to other groups. (40× magnification).