Cardiac VCAM-1 expression and edema. Immunofluorescent staining of VCAM-1 expression in A. non-LPS treated and B. LPS-treated rats (Bar = 25 μm). VCAM-1 expression (green), membranes (WGA, red) and nuclei (DAPI, blue) are visible. C. Fluorescence intensity in arbitrary units (A.U.) was increased after LPS treatment (P = 0.001). D Cardiac wet/dry ratio decreased Vt-dependently (P = 0.001) particularly after LPS treatment (P = 0.029).