Figure 5
S100A7 activates PBMC and neutrophils. (A-D) PBMC were cultured (1 × 106 cells/ml) for 48 and 72 h with or without S100A7. Thereafter, cell culture supernatants were analyzed for levels of IL-6, IL-8 and IL-10 by use of ELISA, and the proliferative capacity of the cells was determined by [methyl-3H]thymidine incorporation (n = 6). (E-H) Neutrophils were cultured (4 × 106 cells/ml) for 4 and 18 h in the absence or presence of S100A7. Levels of IL-6, IL-8 and IL-1β were measured in the supernatants by ELISA, and expression of CD11b was analyzed by flow cytometry (n = 6). Data are expressed as mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (ANOVA).