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Figure 4 | Respiratory Research

Figure 4

From: Oxidative stress induced Interleukin-32 mRNA expression in human bronchial epithelial cells

Figure 4

Transcriptional activity of promoter of IL-32 gene after IFNγ or H 2 O 2 + IFNγ stimulation. HBE cells were transfected with a luciferase promoter vector without mutations (pWild-Luc), closed bars, or the same vector with mutations in the c-Jun/CREB binding site (pMutant-Luc), open bars. Cells were stimulated with H2O2, IFNγ, H2O2 + IFN, or vehicle. Six hours after indicated stimulation, the cells were lysed and luciferase activity was measured. Luciferase activity in the cells was normalized to Renilla luciferase activity. The bars represent the means ± SE from 3 different individuals. *p < 0.05 significantly different.

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