Figure 3

A) Myeloperoxidase (MPO) staining of neutrophils before induction of apoptosis (left panel), alveolar macrophages in BALF from mice that received saline instillation (middle panel) and apoptotic neutrophils phagocytosed by alveolar macrophages in BALF from mice that received apoptotic neutrophils instillation (right panel). B) Number of MPO-positive alveolar macrophages per kilogram of body weight in BALF of mice instilled with 10⁶ apoptotic neutrophils, receiving saline (-) or poractant alfa (+) treatment. C) Phagocytosis index of apoptotic neutrophils ingestion by alveolar macrophage in BALF of mice instilled with 10⁶ apoptotic neutrophils, receiving saline (-) or poractant alfa treatment (+). The phagocytosis index was calculated by multiplying the percentage of MPO-positive alveolar macrophages with the average number of apoptotic neutrophils ingested per MPO-positive alveolar macrophage. Results (n = 11 animals per group) are shown as mean ± SEM. Significant differences (p < 0.05) are marked by *.