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Figure 4 | Respiratory Research

Figure 4

From: Repair of tracheal epithelium by basal cells after chlorine-induced injury

Figure 4

Trichrome staining and α-smooth muscle actin immunohistochemistry. Mice were exposed to chlorine, and tracheas were collected for analysis 7 days after exposure. A. Trichrome staining of distal trachea containing fibrotic lesions. Arrows indicate collagen (blue) staining in fibrotic area. Scale bar represents 100 μm. B. Immunohistochemistry for α-smooth muscle actin in distal trachea showing lack of staining in an area of efficient epithelial repair. C. Immunohistochemistry for α-smooth muscle actin showing staining in a fibrotic region of distal trachea. Arrows indicate areas with α-smooth muscle actin within the fibrotic lesion. Arrowhead shows stronger staining in airway smooth muscle. Scale bars in panels B and C represent 20 μm. Scale bar in panel B represents 20 µm in B and C.

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