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Table 4 Quantitative PCR Validation of Microarray Expression data

From: Gene expression analysis of a murine model with pulmonary vascular remodeling compared to end-stage IPAH lungs

Gene symbol

Gene Name

Fold Change (P value)*

  

RT-PCR

Microarray

Acvrl1

Activin A receptor, type II-like 1

0.7(0.07)

0.7(0.05)

Bmpr2

Bone morphogenic protein receptor, type II

0.6(0.04)

0.8(0.04)

Eng

Endoglin

0.5(0.05)

0.7(0.05)

Ccl8

Chemokine ligand 8

14.0(0.06)

10.3(0.04)

Ccl9

Chemokine ligand 9

9.5(0.03)

11.4(0.02)

Ear11

Eosinophil-associated, ribonuclease A family, member 11

76.0(0.04)

72.4(0.02)

Igj

Immunoglobulin joining chain

20.5(0.26)

10.0(0.05)

Mmp12

Matrix metallopeptidase 12

104.6(0.05)

46.1(0.02)

Mmp13

Matrix metallopeptidase 13

5.1(0.17)

5.7(0.05)

Mmp19

Matrix metallopeptidase 19

3.1(0.05)

2.9(0.05)

Nos3

Nitric oxide synthase 3, endothelial cell

0.7(0.09)

0.8(0.17)

Pdgfa

Platelet derived growth factor, alpha

0.7(0.01)

0.8(0.07)

Vegfa

Vascular endothelial growth factor A

0.6(<0.01)

0.7(0.04)

Gapdh†

Glyceraldehyde-3-phosphate dehydrogenase

1.0(<0.01)

0.9(0.46)

  1. *Fold change values are calculated by dividing the experimental group values by the control values. Statistical analyses were performed by non-paired t-tests
  2. †Gapdh was used as an internal control.