Figure 3
LPS/PAF-induced inflammatory cell influx and airway hyperresponsiveness was comparable between Lp-PLA 2 -/- and WT mice . (A-B): C57BL/6 mice were administered nothing (Naïve, white bars), saline (light grey bars), 1 μg LPS (hatched bars), 1 μg PAF (dotted bars) or 1 μg LPS+PAF (dark grey bars) via intra-tracheal instillation under light anesthesia. BAL was obtained 24 hours later. (A): Cytospin preparations of the cell pellet were assessed under light microscope. Giemsa stained panels are showing representative BAL samples from PAF (left panel), LPS (middle panel) and LPS+PAF (right panel) treated mice. (B): Differential cell count was performed using total cell counts and cytospin preparations. Cells were identified as Macrophages (MP), eosinophils (EP), neutrophils (NP) and lymphocytes (LC). Data are presented as absolute cell number of BAL (mean± SEM, n=6). (C-E): C57BL/6 wild type (WT, white circles) and LP-PLA2-/- (black circles) mice were administered 1 μg LPS+PAF via intra-tracheal instillation under light anesthesia. Lung resistance (C) and lung dynamic compliance (D) were measured by FlexiVent in response to increasing doses of MCh 24 hours after treatment. (E): BAL cell pellet was assessed and data are presented as absolute cell number of BAL (individual data points [n=6-7] are shown with the median indicated by a horizontal line).