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Figure 1 | Respiratory Research

Figure 1

From: The effect of lipoprotein-associated phospholipase A2 deficiency on pulmonary allergic responses in aspergillus fumigatus sensitized mice

Figure 1

Lp-PLA 2 targeting strategy and measurement of PAF-AH activity in mice. (A): Generation of Lp-PLA2-/- mice: Homologous recombination in ES cells was used to generate ES cells heterozygous for the Lp-PLA2 primary targeted allele. Breeding of mice heterozygous for the Lppla2 primary targeted allele with a germline Cre-deleter strain resulted in mice heterozygous for the Lp-PLA2 null allele. Lp-PLA2 exons are indicated as blue boxes; the neor selection cassette as a red box; loxP and FRT sites as yellow and green arrowheads respectively. The positions of the PCR-derived homology arms in relation to the genomic locus structure are indicated by dotted lines. (B): PAF hydrolysis is defective in Lp-PLA2-/- mice. PAF-AH activity in the serum of C57BL/6 wild type (WT) and Lp-PLA2-/- mice was measured as described. Data are expressed as Mean±SEM; n=6-8.

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