Figure 5

Expression of angiogenesis-related genes in CD31⁺cells after pneumonectomy. A-D) Gene expression in mice 3, 6, 14 and 21 days after pneumonectomy was compared to age-matched controls without surgery. The log₂ fold-change in gene expression was plotted against the p-value (t-test) to produce a "volcano plot." The vertical threshold reflected the relative statistical significance (red horizontal line, -log₁₀, p < 0.05); the horizontal threshold reflected the relative fold-change in gene expression (blue vertical line, 4-fold). The significantly up-regulated expression of specific genes (red elipses): a) Tnfaip2, Plg, Anpep, Pgf, Vegfb and Thbs2; b) Csf3, Col4a3, Sphk1, Ereg, Vegfa, Lect1, Col18a1 and Il6; c) Ereg, Ephb4 and Vegfa; d) Tbx1, Cxcl5, Col4a3, Col18a1, F2, Vegfc, Sphk1, Ccl11, S1pr1, F2, Il6, Lect1, Flt1 and Csf3. e) Sphk1 and Vegfa; f) Csf3. Each data point reflects triplicate or quadruplicate arrays of 9 to 21 mice (Day 0, N = 15 mice; Day 3, N = 9 mice; Day 6 = 9 mice; Day 14, N = 9 mice; Day 21, N = 21 mice). E) Indicating a limited impact of the thoracotomy alone, the volcano plot comparison of sham thoracotomy and no surgery control demonstrated no significant change in gene expression on Day 14 after pneumonectomy. F) Scattergram indicating the stability of the control cell population (CD11b^- CD31^- ) used for gene expression analysis. Angiogenesis gene expression in the CD11b^-CD31^- cells was compared on Day 0 and Day 14 after pneumonectomy (N = 3 mice, each time point). Gene expression demonstrated only one gene (Bai1) with significantly decreased expression on Day 14 after pneumonectomy.