Figure 4

Representative gene transcription profile of CD31⁺cells. Baseline (control) lungs were enzymatically digested and the CD31⁺ cells isolated by flow cytometry cell sorting. The RNA extracted from CD31⁺ cells consistently demonstrated RIN greater than 8 (inset: representative RNA electropherogram). The CD31⁺ cells were compared to CD31^-/CD11b^- cells on multiple PCR arrays (PAMM-015, PAMM-011 and PAMM-406, SABiosciences). The CD31⁺ cell gene transcription profile, expressed as the fold regulation (Log₂), was grouped by phenotypic and functional associations: A) leukocyte membrane molecules, B) endothelial cell function-associated molecules, C) extracellular matrix molecules and D) angiogenesis and growth factor molecules. Data representative of N = 16 mice.