Figure 2
From: Angiogenesis gene expression in murine endothelial cells during post-pneumonectomy lung growth
Cell cycle profiling of post-pneumonectomy lung CD31+ endothelial cells obtained by enzymatic digestion and analyzed using flow cytometry (ModFit, Verity Software House). Automated analysis of the ModFit model components, processed by a Marquardt nonlinear least-squares analysis, excluded aggregates (green) and identified S phase (blue hatched area) and G2 phase (second red peak) cells at five time points (Day 0, 3, 6, 14 and 21). The combined analysis of the percentage of cells in S+G2 phase of the cell cycle at various time points after pneumonectomy in the column chart (right; mean ± SD; 4-6 mice per time point).