Rapid translocation of Nrf2 to the nucleus. Increase in levels of Nrf2 in the nuclear protein fraction of cells observed at 2 hr following treatment with orange oil compared to buffer and soy oil-treated controls. Representative blots from one of three separate experiments are shown above. Mean Nrf2 blot densities normalized to those of their corresponding nuclear lamin B for all three experiments presented below. Bars represent mean ± SEM.