Treatment of cells with the mixed oil preparation increases expression of oxidant-protective pathways with differing activation kinetics. Time-courses of expression of antioxidant genes HO-1, NQO1, GCLm, and GCLc in cells of the BEAS-2B human bronchial epithelial line at designated times following the 15 min treatment period. Data are presented as fold change from time-matched soy oil controls after normalization to the expression of actin. Presented are results from three separate experiments. The dashed line indicates the 2-fold level of increased expression as a reference for potential biological significance. Results are expressed as mean ± SEM. * indicates significantly different from time-matched soy oil controls (P < 0.05) by Student's t test.