Figure 6
High tidal volume ventilation caused a time-dependent increase on Akt activation. Western blot was performed using an antibody that recognizes the phosphorylated Akt expression (A and B, top panel) and an antibody that recognizes total Akt protein expression in lung tissue homogenate (A and B, middle panel) from control, nonventilated mice, and mice ventilated with VT 30 ml/kg breathing room air or hyperoxia at indicated time periods. Arbitrary units were expressed as relative Akt phosphorylation (A and B, bottom panel) (n = 5 per group). * P < 0.05 versus control, nonventilated mice. O2 = mice with hyperoxia; RA = mice with room air.