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Figure 5 | Respiratory Research

Figure 5

From: Low-molecular-weight heparin reduces hyperoxia-augmented ventilator-induced lung injury via serine/threonine kinase-protein kinase B

Figure 5

Enoxaparin and Akt deficient mice reduced hyperoxia-augmented lung stretch-induced HMGB1 mRNA expression and Akt activation. Wild-type or Akt+/- mice were ventilated at VT 30 ml/kg for 2 hours with room air or hyperoxia. Enoxaparin, 4 mg/kg, was given subcutaneously 30 minutes before ventilation. (A) RT-PCR assay was performed for HMGB1 mRNA (A, top panel), GAPDH mRNA (A, middle panel), and arbitrary units (A, bottom panel). Arbitrary units were expressed as the ratio of HMGB1 mRNA to GAPDH (n = 5 per group). (B) Western blot was performed using an antibody that recognizes the phosphorylated Akt expression (B, top panel) and an antibody that recognizes total Akt protein expression in lung tissue homogenate (B, middle panel). Arbitrary units were expressed as relative Akt phosphorylation (B, bottom panel) (n = 5 per group). * P < 0.05 versus control, nonventilated mice; † P < 0.05 versus all other groups. Akt+/- = Akt deficient mice; LMWH = enoxaparin; O2 = mice with hyperoxia; RA = mice with room air.

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