Figure 4
High tidal volume ventilation caused a time-dependent increase on HMGB1 mRNA expression. Reverse transcription-polymerase chain reaction (RT-PCR) assay was performed for HMGB1 mRNA (A and B, top panel), glyceraldehydes-phosphate dehydrogenase (GAPDH) mRNA (A and B, middle panel), and arbitrary units (A and B, bottom panel) from control, nonventilated mice and mice ventilated with VT 30 ml/kg breathing room air or hyperoxia at indicated time periods (n = 5 per group). Arbitrary units were expressed as the ratio of HMGB1 mRNA to GAPDH. * P < 0.05 versus control, nonventilated mice. O2 = mice with hyperoxia; RA = mice with room air.