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Figure 1 | Respiratory Research

Figure 1

From: Real time analysis of β2-adrenoceptor-mediated signaling kinetics in Human Primary Airway Smooth Muscle Cells reveals both ligand and dose dependent differences

Figure 1

Isoproterenol-induced changes in cyclic AMP activity in a single hASM cell imaged via confocal microscopy using the altered emission profile of CFP-Epac(dDEP,CD)-VENUS as a readout. CFP-Epac(dDEP,CD)-VENUS expression 48 hours post-transfection is shown in panel A (CFP excited with 440 nm and 480 nm CFP emission fluorescence recorded). Panel B shows uncorrected FRET in the same cell under basal conditions (CFP excited with 440 nm and 535 nm FRET emission fluorescence recorded). Panel C is a representative trace of the altered emission ratio (essentially a change in FRET) in real time in response to 10 μM isoproterenol (+ Iso).

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Respiratory Research

ISSN: 1465-993X

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