Figure 6

FoxA2 induced α ₅ transcription in bronchiolar and alveolar epithelial cell lines. FoxA2 dose-dependently induced α₅ transcription by acting on a 0.85-kb α₅ reporter in Beas-2B and A549 cells (A). FoxA2 and GATA-6 also cooperated to induce a highly significant increase in α₅ transcription in Beas-2B cells (A) but did not elicit a similar increase in A549 cells. Mutagenesis of a single putative FoxA2 response element completely eliminated FoxA2-mediated increases in α₅ transcription and inhibited FoxA2-GATA-6 cooperation in the regulation of α₅ gene expression (B). Significant differences in luciferase levels compared to reporter alone are noted at P ≤ 0.05 (*) and P ≤ 0.01 (**).