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Figure 5 | Respiratory Research

Figure 5

From: Epithelial cell senescence impairs repair process and exacerbates inflammation after airway injury

Figure 5

BrdU induces cellular senescence in Clara-cell-like human lung adenocarcinoma cells. NCI-H441 cells were exposed to BrdU at sublethal concentrations of 25, 50, or 100 μM for 10 days and evaluated for (A) SA β-gal activity, (B) cell cycle progression by flow cytometry, (C) p21 expression by immunoblotting and immunofluorescence, and (D) γH2AX expression by immunofluorescence. (E) Epithelial wound repair in the presence (closed circles) or absence (open circles) of 25 μM BrdU after mechanical damage of NCI-H441 cell monolayers. Data are expressed as the means ± SEM. N = 3-9 in each experiment. *P < 0.05 vs. cells not exposed to BrdU.

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