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Figure 3 | Respiratory Research

Figure 3

From: Epithelial cell senescence impairs repair process and exacerbates inflammation after airway injury

Figure 3

BrdU induces epithelial cell senescence after repeated NA exposure in mice. (A) Lung tissue sections were double stained for γH2AX (green fluorescence) and CC10 (red fluorescence), for phospho-ATM/ATR substrates (brown) and CC10 (green), for p21 (brown) and CC10 (green), or for SA β-gal (green) and CC10 (brown). White arrows indicate γH2AX foci in the nuclei of CC10-positive cells. Black arrows indicate CC10-positive cells that express phospho-ATM/ATR substrates, p21, or SA β-gal. (B) Quantitative analyses of the number of γH2AX foci in CC10-positive cells and the proportion of CC10-positive cells that express phospho-ATM/ATR substrates, p21, or SA β-gal. Data are expressed as the means ± SEM. N = 4-6 in each group of mice. Panel C shows epithelial cells that are double positive for SA β-gal (green) and BrdU (brown) (broken arrows)

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