Figure 3From: Epithelial cell senescence impairs repair process and exacerbates inflammation after airway injuryBrdU induces epithelial cell senescence after repeated NA exposure in mice. (A) Lung tissue sections were double stained for γH2AX (green fluorescence) and CC10 (red fluorescence), for phospho-ATM/ATR substrates (brown) and CC10 (green), for p21 (brown) and CC10 (green), or for SA β-gal (green) and CC10 (brown). White arrows indicate γH2AX foci in the nuclei of CC10-positive cells. Black arrows indicate CC10-positive cells that express phospho-ATM/ATR substrates, p21, or SA β-gal. (B) Quantitative analyses of the number of γH2AX foci in CC10-positive cells and the proportion of CC10-positive cells that express phospho-ATM/ATR substrates, p21, or SA β-gal. Data are expressed as the means ± SEM. N = 4-6 in each group of mice. Panel C shows epithelial cells that are double positive for SA β-gal (green) and BrdU (brown) (broken arrows)Back to article page