Figure 5

CSE-induced transmigration of neutrophils through HUVECs is decreased after pre-incubation with α-CD11b or α-CD18. (A) Prior to the transmigration assay, PMNs were pre-incubated for 20 minutes with medium, α-CD18 (10 μg/ml; upper panel) or α-CD11b (10 μg/ml; lower panel) at 37°C. CSE-induced transmigration of PMNs is decreased after pre-incubation with α-CD11b or α-CD18 (*, p < 0.05 CSE α-CD18 vs. CSE no Ab). FMLP (10^-8 M) was tested as a positive control (⁺, p < 0.01 fMLP vs. control). Legend: each color represents a different donor (N = 4, n = 2). (B) Standardizing the data of figure 5A to an index where the transmigration after CSE incubation is 100%, pre-incubation with α-CD11b or α-CD18 leads to a decrease in transmigration of approximately 35% and 50% resp. (*, p < 0.05; ** p < 0.01 CSE α-CD18/α-CD11b vs. CSE no Ab). Spontaneous transmigration of PMNs was also inhibited by blocking CD11b and CD18 (*, p < 0.05; ** p < 0.01 control α-CD18/α-CD11b vs. control no Ab). FMLP (10^-8 M) was tested as a positive control (*, p < 0.05; ** p < 0.01 fMLP α-CD18/α-CD11b vs. fMLP no Ab).